Department of Biotechnology
Laboratory of Biochemistry and Cell Biology
MAIN DIRECTIONS OF RESEARCH IN THE LABORATORY OF BIOCHEMISTRY AND CELL BIOLOGY
- Analysis of the molecular basis of common and unknown human hereditary hemolytic anemias. The structure and function of red blood cell membrane.
The aim of the research project is the attempt to identify the molecular basis of a hereditary spherocytosis and a new hereditary hemolytic anemia. The team uses such modern research methods as Exome Sequencing and RNA-Seq transcript analysis. These natural mutations underlying hereditary spherocytosis cases in the Polish population are valuable source of knowledge concerning the structure and function of erythrocyte membrane proteins. The subject of research project is also the newly discovered hemolytic anemia diagnosed in the Polish family N associated with the lack of erythrocyte MPP1. The developed recently by our team the effective isolation method of the high quality reticulocyte RNA from peripheral blood is unique and complies with the requirements for high-throughput transcriptome sequencing methods. The association of hemolytic anemias with inherited disorders of bilirubin metabolism result in hyperbilirubinemia and affect the risk of cholelithiasis. Unconjugated hyperbilirubinemia of an extreme degree should raise suspicion of additional factors. Genotyping the UGT1A1 promoter is an important step in the determination of the etiology of free hyperbilirubinemia of unknown origin.
- Study of the role of spectrin in nonerythroid cells.
Nonerythroid spectrin is a membrane skeletal protein involved in the regulation of many cellular processes, including cell adhesion, signaling and actin cytoskeleton remodeling. In this project we investigate a role of αII-spectrin in the immune response and especially its involvement in the different steps required to obtain an efficient immune synapse. In the project we are using molecular, imaging and cellular approaches to show nonerythroid spectrin redistribution upon T cell activation.
- Genetics of animals.
The microsatellite marker is a DNA fragment consisting of repeated two or more nucleotides. Many varieties of such fragments have evolved on the road of evolution. They consist of the same motive but different amount of repetitions. Microsatellite loci are used in many areas, including population genetics. We test the usefulness of selected microsatellite markers is studies identifying Myotis myotis and Plecotus auritus bats and check whether analyzed bat population is in a state of genetic equilibrium. Moreover, we investigate the phylogeographic analyses with the use mitochondrial and nuclear loci of slow worms, legless lizards of the genus Anguis (Squamata: Anguidae).
Laboratory of Biotechnology
MAIN DIRECTIONS OF RESEARCH IN THE BIOTECHNOLOGY LABORATORY
- synthesis, functionalisation and immobilization of selected drugs, and cytotoxicity testing of iron oxide and gold nanoparticles
Research focuses on the chemical and biological synthesis of gold and iron oxide nanoparticles. In the process of green synthesis of metallic nanoparticles, we used biological materials. Our latest research shows that plants and microorganisms have been successfully used in the synthesis of gold nanoparticles.
For selected cell lines, cytotoxicity studies of synthesized iron oxide nanoparticles are carried out after their prior functionalization. Obtained results due to lack or low cytotoxicity indicate the possibility of using these materials in therapy.
- release kinetics of drugs immobilized on the surface of functionalised nanoparticles
Synthesized nanoparticles are functionalized and immobilized with selected drugs for specific drug carriers. For this reason, also the parameters of drug release from the surface of immobilized nanoparticles are tested, as well as the kinetics of their release
- study of the effect of the presence of iron oxide nanoparticles on cultures of lactic bacteria in the aspect of applications in the production of lactic acid
The research is focused on demonstrating the influence of iron(II, III) oxide nanoparticles on microorganisms, mainly on lactic bacteria and on the practical application of the demonstrated effects of nanoparticles on bacteria. Preliminary studies show a positive effect on the survival of lactic bacteria grown in a pH-reduced medium.
- assessment of possible effects on the condition of the body through the intervention of the immune
The subject of the research are organisms with pathophysiological changes for which the indicators of the body’s state in correlation with environmental changes of their presence are analyzed.
- development and experimental verification of new algorithms for the control of bioprocesses in order to simultaneously maximize the efficiency of biomass production and growth rate
New algorithms to control of bioprocesses with the aim of simultaneous maximization of biomass yield and specific growth rate have been established. The new algorithms are implemented in Java and C#. Brocesses controlled by computer are carried out to check the correctness of the algorithms.
- bioproduct (honey) analyst in terms of quality assessment
The suitability of 3D-fluorescence spectra for the identification of individual phenolic acids and flavonoids in analyzed samples of honey of the different botanical origin is discussed in terms of the potential application of 3D-SFS for very fast classification of honey and the detection of its adulterations. A simple, sensitive and specific method based on HPLC-ESI-MS/MS in a negative single reaction ion monitoring (SRM) scan mode ist developed for the detection and quantification of phenolic compounds in Polish honeys and allowed to compare them with other European honeys.